Equine sleep and thoroughbred gene research share award

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Daniela Amiouny’s study set out to explore the factors affecting the duration of sleep stages and whether there was a link between sleep and cognitive performance.
Daniela Amiouny’s study set out to explore the factors affecting the duration of sleep stages and whether there was a link between sleep and cognitive performance.

Research on bedding and light on horse sleep and a study on the myostatin gene in thoroughbreds have jointly earned this year’s British Equestrian Trade Association (BETA) Equine Thesis of the Year Award.

It is the first time there have been joint winners of the award, with Victoria O’Hara, of the Royal Veterinary College, and Daniela Amiouny, of Aberystwyth University, each receiving a trophy and cash prize of £200. They were among the four finalists who presented their theses to the judges online late last month.

The two winners were selected by a judging panel, headed by research consultant Dr Georgina Crossman, with equine nutritionist Katie Williams, vet Karen Coumbe and Equestrian Trade News editor Liz Benwell. Crossman said it had been a challenging year, with students often completing the final stages of their degree in testing lockdown conditions with limited access to resources.

“We congratulate each of the finalists on the exceptional quality of both their dissertations and presentations. They covered a diverse range of topics and we thoroughly enjoyed hearing about their studies in more detail, although it did make it incredibly tough for us to separate them!” Crossman said.

BETA executive director Claire Williams said the judges deliberated for an hour, which showed “just how high the standard of work was and why it was necessary to take the unprecedented step of selecting joint winners”.

Daniela Amiouny
Daniela Amiouny
Equine sleep and memory

Daniela Amiouny, who has completed her undergraduate degree in equine and veterinary bioscience, and is now enrolled at the Canadian Academy of Osteopathy, presented her thesis “The effects of night light and bedding depth on equine sleep and memory consolidation” from her home in Portugal.

Her passion for animal welfare led her to follow this subject. “I was interested to see how husbandry practices – some we don’t even think about, such as leaving the light on in the stable – can affect our horses,” she explained.

Inspired by previous research suggesting that the environment – including bedding depth and type –  along with stable size, might affect horses’ sleep, Amiouny’s study set out to explore the factors affecting the duration of sleep stages and whether there was a link between sleep and cognitive performance.

Using 10 university horses, four different environments were created – a control setting with lights off and 15cm of straw bedding, another with only 5cm of bedding and the lights off, a third with lights on and 15cm of bedding and the final one with lights on and 5cm of bedding. Amiouny then measured the different stages of equine sleep in each 24-hour period over six days, as well as conducting spatial memory tests.

Results showed that suboptimal levels of bedding and lights left on significantly affected both NREM and REM sleep states, and that horses lay down to sleep far more when deeper bedding was used. Amiouny found that memory function did not appear to be impaired by a reduced amount of sleep.

“This could be because the study’s periods of sleep deprivation were not enough or that the actual quality of sleep might not have been affected,” she explained to judges. “Overall, I think the study shows that there are small things that horse owners and carers can do to make a big difference to equine wellbeing.”

Victoria O’Hara
Victoria O’Hara
The myostatin protein

Victoria O’Hara’s study was titled “Use of commercial ELISA for deduction of myostatin protein in equine serum and the examination of an MSTN gene promoter SINE insertion mutation in vivo”.

Myostatin is encoded by one individual gene in thoroughbred and quarter horse DNA. O’Hara’s study looked at the myostatin gene – which prevents muscles from overgrowing – in a series of 185 blood samples previously collected from horses in training at one racing yard. She focused on the mutation in the gene and the effects it has on the levels of myostatin protein found.

Results showed that the horses with the mutation had some myostatin protein, although less than those without the mutation.

“There were also some horses with and without the mutation that didn’t follow this through and there was quite a lot of overlap in the myostatin concentration in blood between groups,” explained O’Hara, who is now working as an equine vet. “Finding out the cause of these outliers and the overlap might help us to discover other performance markers in racehorses and help with myostatin research in other species.”

 

The other finalists were:

Rachel Smith, of University Centre Sparsholt, with her thesis “The effect of breast support on 3D relative breast displacement and upper body muscle activity in female horse riders on an equine simulator”.

Nina Robinson, of Bishop Burton College, with her thesis “A preliminary report of the pressure present beneath bitless and bitted bridles and the effect on equine locomotion”.

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