Influenza D antibodies confirmed in horses in the American Midwest

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Researchers at South Dakota State University have found antibodies against influenza D in blood samples from horses in the Midwest. Photo: South Dakota State University
Researchers at South Dakota State University have found antibodies against influenza D in blood samples from horses in the Midwest. Photo: South Dakota State University

Horses can become infected with influenza D, researchers have confirmed.

South Dakota State University researchers found antibodies against two strains of influenza D in blood samples from horses in the American Midwest.

“It’s nothing we need to worry about at this moment, but more studies are needed,” says Professor Feng Li, from the Department of Biology and Microbiology.

“Horses are clearly another host for the influenza D virus, but we do not know what type of symptoms horses experience.”

In cattle, influenza D typically appears as part of a respiratory disease complex.

The next step in horses will be to isolate the virus and characterize it to determine whether it is different from other strains.

Ben Hause, at Cambridge Technologies in Minnesota, first isolated the virus from a diseased pig in 2011, but later found that cattle were the primary reservoir for the influenza D virus.

Hause identified and characterized those two strains of the virus as part of his doctoral research under the tutelage of Li.

South Dakota State researchers have gone on to identify influenza D antibodies in goats and sheep in the Midwest and have shown that the guinea pig can be used as an animal model to study the virus.

In 2016, the International Committee of Taxonomy of Viruses officially announced a new genus, Orthomyxovirdae, with a single species, Influenza D virus, because of its distinctness from other influenza types — A, B and C.

Analyzing Midwest blood samples

Li and his research team tested 364 blood samples collected in 2015 from horses at 141 farms in Iowa, Minnesota, North Dakota, Nebraska, South Dakota and Wyoming for antibodies to both influenza D strains and the closely related human influenza C.

The farms also have cattle and are in a region where influenza D exposure had been detected.

Blood samples were obtained through the South Dakota Animal Disease and Diagnostic Research Laboratory.

Results showed that 12 percent of the horse blood samples had antibodies against influenza D which has been isolated from pigs and 11 percent had antibodies against the strain isolated from cattle. Furthermore, 11 percent of the horse samples tested positive for human influenza C antibodies.

In addition, the researchers tested 100 blood samples collected from horses and ponies at a farm show in 2016. The animals in this study were young and represented fewer states and a smaller number of farms, Li pointed out. Three tested positive for antibodies against the influenza strain isolated from pigs, while two were positive for the influenza D strain from cattle. Eight animals had antibodies against human influenza C.

Furthermore, 22 blood samples from the 2015 group and 14 from the 2016 group were validated using a more specific, more sensitive test that verifies that the antibodies effectively neutralize the virus, Li explained. The testing confirmed the primary screening findings.

The results have been published in the journal Zoonoses and Public Health.

Though some animals had antibodies to both influenza D and human influenza C, Li says there is no correlation between the two in the study, so far.

Moving forward

Li says the study had shown that when cattle and horses mix, the possibility for virus transmission between the species exists. “The virus can spill over from cattle to horses.”

Whether influenza D can be transmitted among horses has yet to be determined.

Li notes that in one instance, all four horses on the same farm tested positive for influenza D antibodies.

“It may be possible that one horse contracted the virus from cattle and then transmitted it to the other three,” Li said.

He expects to find some level of transmission among horses.

Nedland HWollman JSreenivasan C, et al. Serological evidence for the co-circulation of two lineages of influenza D viruses in equine populations of the Midwest United StatesZoonoses Public Health2018;65:e148e154https://doi.org/10.1111/zph.12423

 

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