A groundbreaking study has found that the environment arising from the traditional farming methods and way of life of the Amish appears to be crucial in conferring protection against asthma.
An interdisciplinary team of researchers probed the differences between two farming communities — the Amish of Indiana and the Hutterites of South Dakota.
The researchers, whose findings have been published in the New England Journal of Medicine, found that specific aspects of the Amish environment were associated with changes to immune cells that appeared to protect children from developing asthma.
The researchers — from the University of Arizona, University of Chicago and the Dr von Hauner Children’s Hospital in Munich, Germany — showed that substances in the house dust from Amish, but not Hutterite, homes were able to engage and shape the innate immune system (the body’s front-line response to most microbes) in young Amish children in ways that may suppress pathologic responses leading to allergic asthma.
The Amish and Hutterite farming communities in the United States, founded by immigrants from Central Europe in the 18th and 19th centuries, respectively, provide textbook opportunities for such comparative studies. The Amish and the Hutterites have similar genetic ancestry. They share similar lifestyles and customs, such as no television and a Germanic farming diet. They have large families, get childhood vaccinations, breastfeed their children, drink raw milk and don’t allow indoor pets.
The communities, however, are distinct in two important ways. Although both groups depend on agriculture, their farming practices differ. The Amish have retained traditional methods. They live on single-family dairy farms and rely on horses for fieldwork and transportation. In contrast, the Hutterites live on large communal farms. They use modern, industrialized farm machinery. This distances young Hutterite children from the constant daily exposure to farm animals.
The other striking difference is what study co-author Carole Ober, professor and chairman of human genetics at the University of Chicago, calls a “whopping disparity in asthma.”
About 5 percent of Amish schoolchildren ages 6 to 14 have asthma. This is about half of the US average (10.3 percent) for children aged 5 to 14, and one-fourth of the prevalence (21.3 percent) among Hutterite children.
To understand this disparity, the researchers studied 30 Amish children aged 7 to 14, and 30 age-matched Hutterite children. They scrutinized the children’s genetic profiles, which confirmed the remarkable similarities between Amish and Hutterite children. They compared the types of immune cells in the children’s blood, collected airborne dust from Amish and Hutterite homes and measured the microbial load in homes in both communities.
The first “gee-whiz” moment came from the blood studies. These revealed startling differences between the innate immune response from the Amish and Hutterites.
“The Amish had more and younger neutrophils, blood cells crucial to fight infections, – and fewer eosinophils, blood cells that promote allergic inflammation,” said immunologist Anne Sperling, an associate professor of medicine at the University of Chicago and a co-author of the study. Gene expression profiles in blood cells also revealed enhanced activation of key innate immunity genes in Amish children.
The second eureka moment came from experiments using mice. When immunologist Donata Vercelli, associate director of the Asthma and Airway Disease Research Center at the University of Arizona Health Sciences, exposed mice to house-dust extracts, she found the airways of mice that received Amish dust were protected from asthma-like responses to allergens. In contrast, mice exposed to Hutterite house dust were not protected.
To better understand how asthma protection was achieved, the researchers used mice that lack the MyD88 and Trif genes which are crucial for innate immune responses. In these mice, the protective effect of the Amish dust was completely lost.
“The results of the mouse experiments conclusively prove that products from the Amish environment are sufficient to confer protection from asthma, and highlight the novel, central role that innate immunity plays in directing this process,” Vercelli said.
What was different? Dust collected from Amish homes was much richer in microbial products, the authors noted, than dust from Hutterite homes.
“Neither the Amish nor the Hutterites have dirty homes,” Ober explained. “Both are tidy. The Amish barns, however, are much closer to their homes. Their children run in and out of them, often barefoot, all day long. There’s no obvious dirt in the Amish homes, no lapse of cleanliness. It’s just in the air, and in the dust.”
“In the end,” the authors concluded, “the novelty of our work lies in the identification of innate immunity as the primary target of the protective Amish environment.”
Co-author Erika von Mutius, a professor at Dr von Hauner Children’s Hospital in Munich, Germany, said: “We hope that our findings will allow the identification of relevant substances that will lead to completely novel strategies to prevent asthma and allergy.”
“You can’t put a cow in every family’s house,” Ober added, “but we may be able to protect children from asthma by finding a way to re-create the time-tested Amish experience.”
Vercelli said the research had proven that the reason the Amish children were so strongly protected from asthma was how they lived.
“We now have a model that over time may allow us to understand what component of the environment is required; basically, we are learning from the Amish environment how to prevent asthma,” she said.
Ober said the work showed that the source of protection was not simply farming. It had narrowed in on what the specific protection might be. “We also clearly show, in humans and in mice, that this protection requires engagement of the innate immune system.”
Additional authors were Justyna Gozdz, Vadim Pivniouk, Julie Ledford, Mauricius Marques dos Santos, Julia Neilson, Sean Murray, Raina Maier and Fernando Martinez, all from the University of Arizona; Michelle Stein, Cara Hrusch, Catherine Igartua and Jack Gilbert from the University of Chicago; and Nervana Metwali and Peter Thorne from the University of Iowa.
Innate Immunity and Asthma Risk in Amish and Hutterite Farm Children
Michelle M. Stein, Cara L. Hrusch, Justyna Gozdz, Catherine Igartua, Vadim Pivniouk, Sean E. Murray, Julie G. Ledford, Mauricius Marques dos Santos, Rebecca L. Anderson, Nervana Metwali, Julia W. Neilson, Raina M. Maier, Jack A. Gilbert, Mark Holbreich, Peter S. Thorne, Fernando D. Martinez, Erika von Mutius, Donata Vercelli, Carole Ober, and Anne I. Sperling.
N Engl J Med 2016; 375:411-421 August 4, 2016DOI: 10.1056/NEJMoa1508749
The study can be read here.
Reporting: University of Arizona