Laser technology sheds light on virus detection

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Mosquitoes like this female Aedes (Ochlerotatus) sp., sucking blood to get nourishment for her eggs, can transmit the viruses that cause West Nile fever and Rift Valley fever.
Mosquitoes like this female Aedes (Ochlerotatus) sp., sucking blood to get nourishment for her eggs, can transmit the viruses that cause West Nile fever and Rift Valley fever.

Lasers could soon be used in tests to rapidly detect disease-causing viruses in animals and humans.

The technology is being developed by United States Department of Agriculture scientists using “surface-enhanced Raman scattering”, or SERS.

With SERS, molecules give off their own unique signals or wavelengths that can be detected with a spectroscope.

Viral molecules can be labeled with a dye that makes them detectable when a laser is shone on them.

Moving a metal such as gold or silver close to the labeled molecules greatly enhances the detection signal.

Microbiologist William Wilson, at the Agricultural Research Service Center for Grain and Animal Health Research in Manhattan, Kansas, used this technology to identify viruses that can cause West Nile fever and Rift Valley fever, both of which are spread by infected mosquitoes.

Wilson and his collaborators at the University of Wyoming designed a nucleic acid diagnostic assay to bring molecules close to gold nanoparticles in solution.

The gold nanoparticles boost the spectroscopic signal from the indicator molecule, making it easier to detect viral nucleic acid from infected cells. They also developed an immunoassay that rapidly detects antibody responses to viruses.

Scientists hope to eventually adapt the assay to field-based bedside or pen-side diagnostic tools.

For example, an instrument similar to a dipstick could be used to rapidly determine areas where a disease outbreak is occurring.

Veterinarians could take blood samples from animals on farms, put the samples in small vials and read them with a hand-held device to determine if a virus is present.

Another advantage of the assay is that it can be used to test for multiple pathogens, whereas current pen-side tests are generally agent-specific.

The sensitivity of the new diagnostic assay is also greater than the current pen-side system and potentially as good as widely used polymerase chain reaction-based tests.

Findings from this research were published in “Biosensors and Bioelectronics and Analytical Chemistry”

 

Reporting: Sandra Avant
Read more about the research in the April 2012 issue of Agricultural Research magazine.

 

 

 

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