The exhibition opens on May 17 and will run until January 4 next year.
It will explore the origin of the species as well as the horse's relationship with humankind, including early interactions between horses and people that led to domestication.
The exhibition will include fossils and cultural objects, ranging from a 19th-century fire engine to paleolithic art.
The American Museum of Natural History was founded in 1869. It has 46 permanent exhibition halls as well as research laboratories and a massive natural history library.
The museum has a permanent collection of more than 30 million specimens and cultural artifacts and a scientific staff of more than 200.